Investigation of Bacterial Outer Membrane Proteins and Suggesting Antiadhesive Agents from Guava Plant in Skin Infections

Abstract

Klebsiella pneumoniae is an opportunistic pathogenic bacterium belonging to Enterobacteriaceae family that owns outer membrane proteins called omp35 and omp36 which are responsible for colonization and adhesion to host tissues such as skin. Twenty pre-diagnosed bacterial isolates of K. pneumoniae of wounds and burn swabs were selected in this study, and the diagnosis was confirmed by VITEK-2 automated system. Whole DNA was isolated then amplified by PCR then gel electrophoresis was performed to detect the prevalence of the genes that encode both of omp35 and omp36 in bacterial isolates. Phylogenetic tree of both studied genes was conducted to investigate the precursor of tested isolates in relation to NCBI data. Virtually, the affinity of two medicinal components of fruit Guava (Psidium guajava L.), which are Quercetin and L-arabinopyranoside, were tested as antiadhesive materials against omp35 and omp35 proteins by molecular docking tools. Results showed that 15 out of 20 skin-infecting K. pneumoniae isolates owned both omp35 and omp36 genes as detected by gel electrophoresis and the phylogenetic trees of both genes illustrated that they were related to surgical and nosocomial swab infection precursors in their origin in NCBI. High affinity of Quercetin was recorded towards omp35 protein and omp36 protein which were (-8.1 and -7.8) respectively, in the same time the affinity of L-arabinopyranoside was (-5.2 and -5.3) towards omp35 and omp36 proteins; resulting in suggesting medicinal ingredients of Guava fruit to prevent adhesion and colonization of K. pneumoniae and other Enterobacteriaceae members which might invade wounded skin by bacterial outer membrane proteins.