Studying of New Bio-Fertilizer Formula Composed of Streptomyces and Nitrogen Fixing Bacteria to Sustain Vigna Radiate (L.)

Abstract

The recent study aimed to develop and produce bio-fertilizer as a supplement to chemical fertilizers, with the goal to reduce their application. In this study, new Streptomyces species were introduced along with nitrogen-fixing bacteria to support the growth of Mung beans in Iraq. The experiment involved the application of five strains of Rhizobium, two strains of Streptomyces, and high indole acetic acid-producing Azospirillum spp4 and Azotobacter spp7. These bio-fertilizers were tested in a field using a complete randomized design at al-Tuwaitha research station in Baghdad.The results indicated that the bio-fertilizer containing Streptomyces and Azospirillum 4 had a significantly positive impact on the growth of Mung bean crops compared to using NPK 100% (conventional chemical fertilizer). The germination percentage reached 75%, with a plant height of 73 cm and 11 branches per plant. The number of legumes per plant was 44.66, with an average legume height of 3.56 cm. The weight of 100 seeds was 142 g, and the fresh weight and dry weight were recorded as 40.33 g and 35.33 g, respectively. Moreover, the study found no statistical difference when comparing the treatment of mung bean seeds with the mono-inoculum of Rhizobium isolated from Phasoleus nodules and Streptomyces Z. This suggests that the newly developed bio-fertilizer containing Streptomyces and Azospirillum 4 is a promising and viable option to enhance the growth and yield of Mung beans without relying heavily on imported chemical fertilizers.